This is the first blog post in a 2 part series in which I will discuss the real-time monitoring of bio-based chemical synthesis.
Biocatalysis continues to evolve, with the application of recombinant organisms or isolated enzymes designed to catalyze specific chemical reactions – often with highly regiospecific and stereospecific conversions. In the optimization of any chemical synthesis reaction – by traditional or biocatalyzed routes – it is not enough to simply report the yield and the enantiameric excess (ee), the kinetics of the reaction must also be considered. Understanding how the kinetics are affected by conditions such as pH and temperature allows optimization of biocatalysis through the identification of operating conditions that can ensure a maximum yield and desired ee in a timely manner. Continue reading